2.Animals Sacrifice and Histologic Samples


The mice were euthanized in CO2 chambers.

  • Incision of the skin with a blade nº 23
  • Finding and isolation of the pretibial muscle using Metzenbaum scissors
  • section of tendons with a Mayo scissors.
  • Fixation of Portions of the muscles on sections of cork oak
  • Embedding using 50% formaldehyde solution - 10% buffered and 50% of pure paraffin for conventional optical microscopic study.
  • Staining Sections as routine histological process.
  • Microtome used : Leika RM 2155
  • Coloration of muscle slices with

-Masson´s trichrome
-and red picro-sirius.

  • Histologic examination was realized with a conventional microscope (Olympus BH-2).
  • Observation under polarized lampe
  • Photographic documentation was realized with a Leika DMR microscope joined to a digital camera Leika CD 300F and compatible PC.