3.Material

boxes rats   6 boxes with 5 rats/ box 

  • Animals were divided in 6 boxes, so that boxes from number 1 to 5 had 5 rats and box number 6 had 4 rats 
  • All animals were submitted to a walking evaluation by footprint impression on special paper , and previous immersion of back legs on a soap solution. 
  • Box 1 had the control group, that received the application of 0,1 ml saline solution ( NaCl 0,9%) on the right pretibial muscle. 

  • The mice of boxes 2 to 5 received 0,1 ml of Endopeels Carbolic Acid  on the same muscle as control group.

  • Box 6 was constituted of 4 rats, and each one received 0,5 ml of Endopeels carbolic acid injected on the subcutaneous layer. 

  • All the animals were submitted to walking evaluation before and after their respective injections.